![]() ![]() ![]() NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED). DNA Polymerase I Large (Klenow) Fragment is a 68kDa C-terminal fragment of E. Our mission is to develop high-quality innovative tools and services to accelerate discovery.įOR RESEARCH USE ONLY. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. (1983) Construction of a plasmid that overproduces the large proteolytic fragment(Klenow fragment) of DNA polymerase I of Escherichia coli. Find, read and cite all the research you. KlenowFragment is free from intact DNA polymerase and small fragment as indicated by SDS-polyacrylamide gel electrophoresis. Klenow Fragment is used primarily in dideoxy sequencing reactions (1,2), fill-in of restriction endonuclease termini (3), and second-strand cDNA synthesis (4). provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. PDF DNA polymerases are an essential class of enzymes or molecular motors that catalyze processive DNA syntheses during DNA replications. ![]()
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